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Virology

• Sierra S., Davila M., Lowenstein P.R., Domingo E. Response of foot-and-mouth disease virus to increased mutagenesis: influence of viral load and fitness in loss of infectivity. Journal of Virolology, 74: 8316-23, 2000. Click here for the PDF

• Pariente, N., Sierra, S., Lowenstein, P.R., and Domingo, E. Efficient virus extinction by combinations of a mutagen and antiviral inhibitors. Journal of Virology, 75: 9723-30, 2001. Click here for the PDF

• Grande-Perez, A., Sierra, S., Castro, M.G., Domingo, E., and Lowenstein, P.R. Molecular indetermination in the transition to error catastrophe. Systemic elimination of lymphocytic choriomeningitis virus through mutagenesis does not correlate linearly with large increases in mutant spectrum complexity. Proceedings of the National Academy of Sciences, USA (2002, In press). Click here for the PDF

• Thomas, CE, Abordo-Adesida, E, Maleniak, TC, Stone, D, Gerdes, G. and Lowenstein, P.R. "Gene transfer into rat brain using adenoviral vectors". Curr Prot In NeuroSci 4.24.1-4.24.36, 2000. Click here for the PDF

• Southgate, T., Kingston, P. and Castro, M.G. Adenoviral vectors for gene transfer into neural cells in primary culture. In Current Protocols in Neuroscience, Ed. D. R. Sibley, J Wiley & Sons, Publishers, 4.23.1 - 4.23.40, 2001.

• Thomas, C.E., Edwards, P., Wickham, T.J., Castro, M.G., Lowenstein, P.R. (2002) Adenovirus binding to coxsackievirus and adenovirus receptor or integins is not required to elicit brain inflammation but is necessary to transduce specific neural cell types. Journal of Virology, 76: 3452-3460. Click here for the PDF

• Umana, P., C. A. Gerdes, D. Stone, J. R. Davis, D. Ward, M. G. Castro and P. R. Lowenstein (2001). "Efficient FLPe recombinase enables scalable production of helper-dependent adenoviral vectors with negligible helper-virus contamination." Nat Biotechnol 19(6): 582-5. Click here for the PDF

• Thomas CE, Schiedner G, Kochanek S, Castro MG, Lowenstein PR."Preexisting antiadenoviral immunity is not a barrier to efficient and stable transduction of the brain, mediated by novel high-capacity adenovirus vectors."
  Hum Gene Ther. 2001 May 1;12(7):839-46. Click here for the PDF

• Lowenstein PR, Thomas CE, Umana P, Gerdes CA, Verakis T, Boyer O, Tondeur S, Klatzmann D, Castro MG."High-capacity, helper-dependent, "gutless" adenoviral vectors for gene transfer into brain. Methods Enzymol. 2002;346:292-311" Click here for the PDF

• Lowenstein PR."Virology and immunology of gene therapy, or virology and immunology of high MOI infection with defective viruses."
  Gene Ther. 2003 Jun;10(11):933-4. Click here for the PDF

• Grande-Perez A, Sierra S, Castro MG, Domingo E, Lowenstein PR."Molecular indetermination in the transition to error catastrophe: systematic elimination of lymphocytic choriomeningitis virus through mutagenesis does not correlate linearly with large increases in mutant spectrum complexity."
  Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12938-43. Epub 2002 Sep 5. Click here for the PDF

• Lowenstein PR, Suwelack D, Hu J, Yuan X, Jimenez-Dalmaroni M, Goverdhana S, Castro MG."Nonneurotropic adenovirus: a vector for gene transfer to the brain and gene therapy of neurological disorders."
  Int Rev Neurobiol. 2003;55:3-64. Click here for the PDF

• Dorigo O, Gil JS, Gallaher SD, Tan BT, Castro MG, Lowenstein PR, Calos MP, Berk AJ."Development of a novel helper-dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cells."J Virol. 2004 Jun;78(12):6556-66 Click here for the PDF

• Lowenstein PR."Input virion proteins: cryptic targets of antivector immune responses in preimmunized subjects." Mol Ther. 2004 Jun;9(6):771-4 Click here for the PDF

• Domingo E, Pariente N, Airaksinen A, Gonzalez-Lopez C, Sierra S, Herrera M, Grande-Perez A, Lowenstein PR, Manrubia SC, Lazaro E, Escarmis C."Foot-and-mouth disease virus evolution: exploring pathways towards virus extinction."Curr Top Microbiol Immunol. 2005;288:149-73

• Abordo-Adesida E, Follenzi A, Barcia C, Sciascia S, Castro MG, Naldini L, Lowenstein PR."Stability of lentiviral vector-mediated transgene expression in the brain in the presence of systemic antivector immune responses."Hum Gene Ther. 2005 Jun;16(6):741-51. Click here for the PDF

• Grande-Perez A, Gomez-Mariano G, Lowenstein PR, Domingo E."Mutagenesis-induced, large fitness variations with an invariant arenavirus consensus genomic nucleotide sequence." J Virol. 2005 Aug;79(16):10451-9. Click here for the PDF

• Xiong W, Goverdhana S, Sciascia SA, Candolfi M, Zirger JM, Barcia C, Curtin JF, King GD, Jaita G, Liu C, Kroeger K, Agadjanian H, Medina-Kauwe L, Palmer D, Ng P, Lowenstein PR, Castro MG."Regulatable gutless adenovirus vectors sustain inducible transgene expression in the brain in the presence of an immune response against adenoviruses." J Virol. 2006 Jan;80(1):27-37. Click here for the PDF

• Grande-Perez A., Lazaro E., Lowenstein P.R., Domingo E., Manrubia S.C. (2005) Suppression of viral infectivity through lethal defection. Proc. Natl. Acad. Sci. USA 102: 4448-4452.

• Iannacone M., Sitia G., Isogawa M., Marchese P., Castro M.G., Lowenstein P.R., Chisari F.V., Ruggeri ZM, Guidotto L.G., Platelets mediate CTL-induced liver damage. Nature Medicine 11(11):1167-1169. Click here for the PDF

• Candolfi M, Kroeger KM, Pluhar GE, Bergeron J, Puntel M, Curtin JF, McNiel EA, Freese AB, Ohlfest JR, Moore P, Lowenstein PR, Castro MG. Adenoviral-mediated gene transfer into the canine brain in vivo. Neurosurgery. 2007 Jan;60(1):167-77 Click here for the PDF

• Candolfi M, Pluhar GE, Kroeger K, Puntel M, Curtin J, Barcia C, Muhammad AK, Xiong W, Liu C, Mondkar S, Kuoy W, Kang T, McNeil EA, Freese AB, Ohlfest JR, Moore P, Palmer D, Ng P, Young JD, Lowenstein PR, Castro MG. Optimization of adenoviral vector-mediated transgene expression in the canine brain in vivo, and in canine glioma cells in vitro. Neuro Oncol. 2007 Jul;9(3):245-58. Click here for the PDF

• Barcia C, Jimenez-Dalmaroni M, Kroeger KM, Puntel M, Rapaport AJ, Larocque D, King GD, Johnson SA, Liu C, Xiong W, Candolfi M, Mondkar S, Ng P, Palmer D, Castro MG, Lowenstein PR. Mol Ther. 2007 Dec;15(12):2154-2163 Click here for the PDF

• Lowenstein PR, Kroeger K, Castro MG. Immunology of neurological gene therapy: how T cells modulate viral vector-mediated therapeutic transgene expression through immunological synapses. Neurotherapeutics. 2007 Oct;4(4):715-24. Review Click here for the PDF

• Lowenstein PR, Mandel RJ, Xiong WD, Kroeger K, Castro MG. Immune Responses to Adenovirus and Adeno-Associated Vectors Used for Gene Therapy of Brain Diseases: The Role of Immunological Synapses in Understanding the Cell Biology of Neuroimmune Interactions. Curr Gene Ther. 2007 Oct;7(5):347-360 Click here for the PDF

• Barcia C, Gerdes C, Xiong WD, Thomas CE, Liu C, Kroeger KM, Castro MG, Lowenstein PR. Immunological thresholds in neurological gene therapy: highly efficient elimination of transduced cells might be related to the specific formation of immunological synapses between T cells and virus-infected brain cells. Neuron Glia Biol. 2007 Jul 13;(2):309-322. Click here for the PDF

• Barcia Carlos, Kolja Wawrowsky, Robert J. Barrett, Chunyan Liu, Maria G. Castro,
  and Pedro R. Lowenstein. In Vivo Polarization of IFN-
  at Kupfer and Non-Kupfer Immunological Synapses during the Clearance of Virally Infected Brain Cells.
  J Immunol. 2008 Feb 1;180(3):1344-1352 Click here for the PDF

• Xiong W, Candolfi M, Kroeger KM, Puntel M, Mondkar S, Larocque D, Liu C, Curtin JF, Palmer D, Ng P, Lowenstein PR, Castro MG. Immunization Against the Transgene but not the TetON Switch Reduces Expression From Gutless Adenoviral Vectors in the Brain. Mol Ther. 2008 Feb;16(2):343-351. Click here for the PDF

• King GD, Muhammad AK, Xiong W, Kroeger KM, Puntel M, Larocque D, Palmer D, Ng P, Lowenstein PR, Castro MG. High-capacity adenovirus vector-mediated anti-glioma gene therapy in the presence of systemic antiadenovirus immunity. J Virol. 2008 May;82(9):4680-4. Epub 2008 Feb 20. Click here for the PDF

• Barcia C, Wawrowsky K, Barrett RJ, Liu C, Castro MG, Lowenstein PR. In vivo polarization of IFN-gamma at Kupfer and non-Kupfer immunological synapses during the clearance of virally infected brain cells. J Immunol. 2008 Feb 1;180(3):1344-52. Click here for the PDF